No. This is for emulators only. Playing burned discs on a real PS2 requires a modchip or FreeDVDBoot, not a BIOS file.
Do not use the 90006 BIOS for older games like Metal Gear Solid 2 or Gran Turismo 3 . Those games were coded before this BIOS existed and sometimes have minor timing glitches. Keep a 39001 (USA) or 50000 (JPN) BIOS for legacy titles. all ps2 bios files including the new scph90006 link
The 90006 BIOS file size should be exactly 4,194,304 bytes (4MB). If it’s smaller, you dumped it incorrectly. 304 bytes (4MB). If it’s smaller